Digital image processing is a technology that processes and analyzes related functions through image data. In the STORM (Stochastic Optical Reconstruction Microscopy) system, the biological sample information is amplified through the optical path, projected onto the main camera sensor plate of the system, converted into digital image data, and processed by the digital image processing system. During this process, both system electronic and environmental noise can affect the imaging. This article describes the active locking function of iSTORM, the ultra-high resolution microscopy imaging system now released by Force Intelligence, which can reduce the impact of external noise on imaging.
01 Why lock?
What is locking? As the name suggests, it is to fix the field of view at a certain point. iSTORM ultra-high imaging system is nanoscale high-precision imaging, slight vibration may cause imaging shift or out of focus, especially when conducting long-term observation experiments, physical vibration will lead to image shift, resulting in inconsistent samples observed before and after the experiment, seriously affecting the quality of the experiment and the experimental conclusion. If the field of view can be locked, the efficiency and quality of biological observation experiments will be greatly improved.
So, how exactly is the active locking technology of the iSTORM ultra-high imaging system realized, and what is the imaging effect? Let me introduce them to you one by one.
02 Implementation of active locking
The essence of the iSTORM ultra-high imaging system is to keep the sample microscope objective relatively fixed, so as to keep the observation field fixed, and the entire calculation process of the iSTORM ultra-high imaging system active locking is only a few milliseconds.
Active lock-in effectiveness is mainly affected by two factors:
(1) the accuracy of the image algorithm;
(2) Response speed and displacement accuracy of the displacement platform;
In image processing, pixel-level recognition and calculation errors are small, and the locking effect is mainly affected by the mobile platform hardware. For example, the weight of the sample, the speed of the stepping motor driving the platform movement of the sample and the accuracy of the mechanical gear, there will be a certain error in the locking process, and a large number of experiments are required to use algorithms to eliminate the displacement error on the hardware.
03 iSTORM Book a test shoot
iSTORM, the ultra-high-resolution microscopic imaging system released by Lixian, has successfully achieved a breakthrough in the diffraction limit of optical microscopy, making it possible to engage in the research of single-molecule localization and counting of biological macromolecules, subcellular and macromolecular complex structure analysis, and biodynamics of biological macromolecules on the resolution scale of 20 nm, thus bringing major breakthroughs to life sciences, medicine and other fields.
The ultra-high resolution microscopy imaging system iSTORM has the characteristics of 20nm ultra-high resolution, 3D imaging, multi-channel simultaneous imaging optical path design, real-time reconstruction, and 2-hour novice mastery, and has realized the localization and counting of single molecules of living cells, and provides fluorescent dye selection, sample preparation, imaging services and experimental solutions as an overall solution, with excellent characteristics such as nanoscale observation accuracy, high stability, wide environmental applicability, rapid imaging, and simple operation. It has been highly recognized by more than 50 scientific research groups and more than 100 researchers.
References
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About us
Ningbo Lixian Intelligent Technology Co., Ltd. (INVIEW) is a scientific and technological enterprise specializing in ultra-high resolution microscopy technology and product research and development, relying on the automatic control, new generation information technology of Fudan University and the biology, optics, image processing and other technologies of the Hong Kong University of Science and Technology, with optics, biology, automatic control, machinery, information technology and other interdisciplinary technical teams, the 2014 Nobel Prize in Chemistry technology industrialization, launched the ultra-high resolution microscopy imaging system iSTORM, A series of products such as the cell intelligent monitoring assistant Celeston Micro help people observe the microscopic world from an unprecedented perspective, break through the limit, and see things like never before.